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        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        This report was generated using MultiQC, version 1.13

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        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2022-11-15, 12:12 AEDT based on data in:

        General Statistics

        Showing 232/232 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        401954_ITS_KP69D_GAAGAAGC-ACGACGTG_S1_L001_R2
        85.2%
        43%
        0.1
        401955_ITS_KP69D_GAAGAAGC-ATATACAC_S2_L001_R2
        79.5%
        45%
        0.1
        401956_ITS_KP69D_GAAGAAGC-CGTCGCTA_S3_L001_R2
        80.3%
        47%
        0.0
        401957_ITS_KP69D_GAAGAAGC-CTAGAGCT_S4_L001_R2
        82.0%
        45%
        0.1
        401958_ITS_KP69D_GAAGAAGC-GCTCTAGT_S5_L001_R2
        80.5%
        47%
        0.1
        401959_ITS_KP69D_GAAGAAGC-GACACTGA_S6_L001_R2
        78.2%
        46%
        0.1
        401960_ITS_KP69D_GAAGAAGC-TGCGTACG_S7_L001_R2
        82.3%
        48%
        0.1
        401961_ITS_KP69D_GAAGAAGC-TAGTGTAG_S8_L001_R2
        78.1%
        45%
        0.0
        401962_ITS_KP69D_GAGAGAAT-ACGACGTG_S9_L001_R2
        78.7%
        51%
        0.0
        401963_ITS_KP69D_GAGAGAAT-ATATACAC_S10_L001_R2
        83.9%
        49%
        0.1
        401964_ITS_KP69D_GAGAGAAT-CGTCGCTA_S11_L001_R2
        83.9%
        47%
        0.1
        401965_ITS_KP69D_GAGAGAAT-CTAGAGCT_S12_L001_R2
        83.9%
        47%
        0.1
        401966_ITS_KP69D_GAGAGAAT-GCTCTAGT_S13_L001_R2
        83.4%
        49%
        0.1
        401967_ITS_KP69D_GAGAGAAT-GACACTGA_S14_L001_R2
        83.8%
        47%
        0.2
        401968_ITS_KP69D_GAGAGAAT-TGCGTACG_S15_L001_R2
        83.8%
        46%
        0.1
        401969_ITS_KP69D_GAGAGAAT-TAGTGTAG_S16_L001_R2
        82.9%
        47%
        0.1
        401970_ITS_KP69D_TCGTCGAT-ACGACGTG_S17_L001_R2
        81.9%
        43%
        0.1
        401971_ITS_KP69D_TCGTCGAT-ATATACAC_S18_L001_R2
        82.4%
        46%
        0.1
        401972_ITS_KP69D_TCGTCGAT-CGTCGCTA_S19_L001_R2
        84.6%
        48%
        0.1
        401973_ITS_KP69D_TCGTCGAT-CTAGAGCT_S20_L001_R2
        83.6%
        47%
        0.1
        401974_ITS_KP69D_TCGTCGAT-GCTCTAGT_S21_L001_R2
        84.3%
        49%
        0.1
        401975_ITS_KP69D_TCGTCGAT-GACACTGA_S22_L001_R2
        82.2%
        47%
        0.1
        401976_ITS_KP69D_TCGTCGAT-TGCGTACG_S23_L001_R2
        81.3%
        47%
        0.1
        401977_ITS_KP69D_TCGTCGAT-TAGTGTAG_S24_L001_R2
        80.2%
        46%
        0.0
        401978_ITS_KP69D_ATTGGGCT-ACGACGTG_S25_L001_R2
        83.2%
        48%
        0.1
        401979_ITS_KP69D_ATTGGGCT-ATATACAC_S26_L001_R2
        81.3%
        47%
        0.1
        401980_ITS_KP69D_ATTGGGCT-CGTCGCTA_S27_L001_R2
        79.6%
        48%
        0.1
        401981_ITS_KP69D_ATTGGGCT-CTAGAGCT_S28_L001_R2
        81.0%
        47%
        0.1
        401982_ITS_KP69D_ATTGGGCT-GCTCTAGT_S29_L001_R2
        80.8%
        47%
        0.1
        401983_ITS_KP69D_ATTGGGCT-GACACTGA_S30_L001_R2
        79.4%
        46%
        0.1
        401984_ITS_KP69D_ATTGGGCT-TGCGTACG_S31_L001_R2
        79.5%
        46%
        0.1
        401985_ITS_KP69D_ATTGGGCT-TAGTGTAG_S32_L001_R2
        78.6%
        46%
        0.1
        401986_ITS_KP69D_ACCACATA-ACGACGTG_S33_L001_R2
        82.6%
        47%
        0.1
        401987_ITS_KP69D_ACCACATA-ATATACAC_S34_L001_R2
        81.1%
        48%
        0.1
        401988_ITS_KP69D_ACCACATA-CGTCGCTA_S35_L001_R2
        81.7%
        48%
        0.1
        401989_ITS_KP69D_ACCACATA-CTAGAGCT_S36_L001_R2
        83.7%
        47%
        0.1
        401990_ITS_KP69D_ACCACATA-GCTCTAGT_S37_L001_R2
        82.5%
        47%
        0.1
        401991_ITS_KP69D_ACCACATA-GACACTGA_S38_L001_R2
        84.2%
        48%
        0.1
        401992_ITS_KP69D_ACCACATA-TGCGTACG_S39_L001_R2
        82.6%
        47%
        0.1
        401993_ITS_KP69D_ACCACATA-TAGTGTAG_S40_L001_R2
        79.0%
        45%
        0.0
        401994_ITS_KP69D_AACACAAG-ACGACGTG_S41_L001_R2
        82.3%
        48%
        0.1
        401995_ITS_KP69D_AACACAAG-ATATACAC_S42_L001_R2
        86.4%
        49%
        0.7
        401996_ITS_KP69D_AACACAAG-CGTCGCTA_S43_L001_R2
        83.3%
        47%
        0.1
        401997_ITS_KP69D_AACACAAG-CTAGAGCT_S44_L001_R2
        80.6%
        47%
        0.2
        401998_ITS_KP69D_AACACAAG-GCTCTAGT_S45_L001_R2
        88.2%
        43%
        0.9
        401999_ITS_KP69D_AACACAAG-GACACTGA_S46_L001_R2
        79.2%
        49%
        0.1
        402000_ITS_KP69D_AACACAAG-TGCGTACG_S47_L001_R2
        82.2%
        48%
        0.1
        402001_ITS_KP69D_AACACAAG-TAGTGTAG_S48_L001_R2
        85.5%
        45%
        0.7
        402002_ITS_KP69D_AATGTCCG-ACGACGTG_S49_L001_R2
        83.2%
        48%
        0.1
        402003_ITS_KP69D_AATGTCCG-ATATACAC_S50_L001_R2
        83.5%
        46%
        0.1
        402004_ITS_KP69D_AATGTCCG-CGTCGCTA_S51_L001_R2
        81.7%
        48%
        0.1
        402005_ITS_KP69D_AATGTCCG-CTAGAGCT_S52_L001_R2
        79.6%
        46%
        0.1
        402006_ITS_KP69D_AATGTCCG-GCTCTAGT_S53_L001_R2
        81.7%
        47%
        0.1
        402007_ITS_KP69D_AATGTCCG-GACACTGA_S54_L001_R2
        80.0%
        47%
        0.1
        402008_ITS_KP69D_AATGTCCG-TGCGTACG_S55_L001_R2
        84.2%
        46%
        0.1
        402009_ITS_KP69D_AATGTCCG-TAGTGTAG_S56_L001_R2
        80.1%
        46%
        0.1
        402010_ITS_KP69D_TACTTCGC-ACGACGTG_S57_L001_R2
        79.7%
        46%
        0.1
        402011_ITS_KP69D_TACTTCGC-ATATACAC_S58_L001_R2
        78.9%
        47%
        0.1
        402012_ITS_KP69D_TACTTCGC-CGTCGCTA_S59_L001_R2
        81.6%
        49%
        0.1
        402013_ITS_KP69D_TACTTCGC-CTAGAGCT_S60_L001_R2
        85.8%
        44%
        0.8
        402014_ITS_KP69D_TACTTCGC-GCTCTAGT_S61_L001_R2
        81.3%
        48%
        0.1
        402015_ITS_KP69D_TACTTCGC-GACACTGA_S62_L001_R2
        79.7%
        48%
        0.1
        402016_ITS_KP69D_TACTTCGC-TGCGTACG_S63_L001_R2
        81.5%
        48%
        0.1
        402017_ITS_KP69D_TACTTCGC-TAGTGTAG_S64_L001_R2
        79.1%
        47%
        0.1
        402018_ITS_KP69D_GCTTCGGT-ACGACGTG_S65_L001_R2
        83.7%
        50%
        0.0
        402019_ITS_KP69D_GCTTCGGT-ATATACAC_S66_L001_R2
        83.8%
        47%
        0.1
        402020_ITS_KP69D_GCTTCGGT-CGTCGCTA_S67_L001_R2
        22.9%
        48%
        0.0
        402021_ITS_KP69D_GCTTCGGT-CTAGAGCT_S68_L001_R2
        85.0%
        46%
        0.1
        402022_ITS_KP69D_GCTTCGGT-GCTCTAGT_S69_L001_R2
        70.3%
        47%
        0.0
        402023_ITS_KP69D_GCTTCGGT-GACACTGA_S70_L001_R2
        85.2%
        48%
        0.1
        402024_ITS_KP69D_GCTTCGGT-TGCGTACG_S71_L001_R2
        84.8%
        48%
        0.1
        402025_ITS_KP69D_GCTTCGGT-TAGTGTAG_S72_L001_R2
        81.2%
        47%
        0.1
        402026_ITS_KP69D_CTTACACC-ACGACGTG_S73_L001_R2
        84.8%
        51%
        0.1
        402027_ITS_KP69D_CTTACACC-ATATACAC_S74_L001_R2
        83.0%
        49%
        0.1
        402028_ITS_KP69D_CTTACACC-CGTCGCTA_S75_L001_R2
        87.9%
        51%
        0.2
        402029_ITS_KP69D_CTTACACC-CTAGAGCT_S76_L001_R2
        85.4%
        48%
        0.4
        402030_ITS_KP69D_CTTACACC-GCTCTAGT_S77_L001_R2
        82.3%
        49%
        0.1
        402031_ITS_KP69D_CTTACACC-GACACTGA_S78_L001_R2
        81.9%
        48%
        0.2
        402032_ITS_KP69D_CTTACACC-TGCGTACG_S79_L001_R2
        84.1%
        49%
        0.1
        402033_ITS_KP69D_CTTACACC-TAGTGTAG_S80_L001_R2
        82.2%
        46%
        0.1
        402034_ITS_KP69D_TGACCTCC-ACGACGTG_S81_L001_R2
        81.4%
        52%
        0.1
        402035_ITS_KP69D_TGACCTCC-ATATACAC_S82_L001_R2
        83.4%
        46%
        0.3
        402036_ITS_KP69D_TGACCTCC-CGTCGCTA_S83_L001_R2
        84.2%
        46%
        0.1
        402037_ITS_KP69D_TGACCTCC-CTAGAGCT_S84_L001_R2
        80.7%
        47%
        0.1
        402038_ITS_KP69D_TGACCTCC-GCTCTAGT_S85_L001_R2
        78.4%
        48%
        0.1
        402039_ITS_KP69D_TGACCTCC-GACACTGA_S86_L001_R2
        80.4%
        48%
        0.1
        402040_ITS_KP69D_TGACCTCC-TGCGTACG_S87_L001_R2
        82.8%
        48%
        0.1
        402041_ITS_KP69D_TGACCTCC-TAGTGTAG_S88_L001_R2
        86.6%
        43%
        0.6
        402042_ITS_KP69D_ACAAGGAG-ACGACGTG_S89_L001_R2
        81.7%
        46%
        0.1
        402043_ITS_KP69D_ACAAGGAG-ATATACAC_S90_L001_R2
        87.3%
        46%
        0.3
        402044_ITS_KP69D_ACAAGGAG-CGTCGCTA_S91_L001_R2
        83.8%
        50%
        0.1
        402045_ITS_KP69D_ACAAGGAG-CTAGAGCT_S92_L001_R2
        84.7%
        45%
        0.5
        402046_ITS_KP69D_ACAAGGAG-GCTCTAGT_S93_L001_R2
        83.3%
        51%
        0.1
        402047_ITS_KP8LM_TCTGTTGC-ACGACGTG_S1_L001_R2
        84.8%
        46%
        0.3
        402048_ITS_KP8LM_TCTGTTGC-ATATACAC_S2_L001_R2
        84.7%
        47%
        0.1
        402049_ITS_KP8LM_TCTGTTGC-CGTCGCTA_S3_L001_R2
        85.0%
        47%
        0.1
        402050_ITS_KP8LM_GAAGAAGC-ACGACGTG_S4_L001_R2
        83.1%
        48%
        0.0
        402051_ITS_KP8LM_GACTCTTG-ACGACGTG_S5_L001_R2
        89.1%
        44%
        0.3
        402052_ITS_KP8LM_GAAGAAGC-CGTCGCTA_S6_L001_R2
        86.0%
        50%
        0.1
        402053_ITS_KP8LM_GAAGAAGC-CTAGAGCT_S7_L001_R2
        85.9%
        47%
        0.1
        402054_ITS_KP8LM_GAAGAAGC-GCTCTAGT_S8_L001_R2
        83.9%
        48%
        0.0
        402055_ITS_KP8LM_GAAGAAGC-GACACTGA_S9_L001_R2
        83.5%
        47%
        0.1
        402056_ITS_KP8LM_GAAGAAGC-TGCGTACG_S10_L001_R2
        86.3%
        47%
        0.1
        402057_ITS_KP8LM_GACTCTTG-ATATACAC_S11_L001_R2
        91.7%
        50%
        0.4
        402058_ITS_KP8LM_GAGAGAAT-ACGACGTG_S12_L001_R2
        83.3%
        47%
        0.0
        402059_ITS_KP8LM_GACTCTTG-CGTCGCTA_S13_L001_R2
        84.5%
        47%
        0.1
        402060_ITS_KP8LM_GAGAGAAT-CGTCGCTA_S14_L001_R2
        82.0%
        47%
        0.0
        402061_ITS_KP8LM_GAGAGAAT-CTAGAGCT_S15_L001_R2
        88.0%
        42%
        0.2
        402062_ITS_KP8LM_GAGAGAAT-GCTCTAGT_S16_L001_R2
        83.8%
        47%
        0.1
        402063_ITS_KP8LM_GAGAGAAT-GACACTGA_S17_L001_R2
        88.6%
        42%
        0.3
        402064_ITS_KP8LM_GAGAGAAT-TGCGTACG_S18_L001_R2
        87.3%
        50%
        0.1
        402065_ITS_KP8LM_GAGAGAAT-TAGTGTAG_S19_L001_R2
        90.4%
        46%
        0.3
        402066_ITS_KP8LM_TCGTCGAT-ACGACGTG_S20_L001_R2
        83.4%
        49%
        0.1
        402067_ITS_KP8LM_TCGTCGAT-ATATACAC_S21_L001_R2
        87.7%
        45%
        0.4
        402068_ITS_KP8LM_TCGTCGAT-CGTCGCTA_S22_L001_R2
        80.6%
        48%
        0.0
        402069_ITS_KP8LM_TCGTCGAT-CTAGAGCT_S23_L001_R2
        88.0%
        47%
        0.3
        402070_ITS_KP8LM_TCGTCGAT-GCTCTAGT_S24_L001_R2
        90.1%
        46%
        0.3
        402071_ITS_KP8LM_GACTCTTG-GCTCTAGT_S25_L001_R2
        87.5%
        43%
        0.3
        402072_ITS_KP8LM_TCGTCGAT-TGCGTACG_S26_L001_R2
        85.2%
        46%
        0.1
        402073_ITS_KP8LM_GACTCTTG-GACACTGA_S27_L001_R2
        88.2%
        48%
        0.2
        402074_ITS_KP8LM_ATTGGGCT-ACGACGTG_S28_L001_R2
        84.6%
        46%
        0.1
        402075_ITS_KP8LM_GACTCTTG-TGCGTACG_S29_L001_R2
        85.9%
        47%
        0.2
        402076_ITS_KP8LM_ATTGGGCT-CGTCGCTA_S30_L001_R2
        84.3%
        49%
        0.1
        402077_ITS_KP8LM_GACTCTTG-TAGTGTAG_S31_L001_R2
        86.7%
        44%
        0.1
        402078_ITS_KP8LM_ATTGGGCT-GCTCTAGT_S32_L001_R2
        82.5%
        47%
        0.0
        402079_ITS_KP8LM_ATTGGGCT-GACACTGA_S33_L001_R2
        83.8%
        50%
        0.1
        402080_ITS_KP8LM_ATTGGGCT-TGCGTACG_S34_L001_R2
        80.1%
        49%
        0.0
        402081_ITS_KP8LM_GCAGGATA-ACGACGTG_S35_L001_R2
        86.1%
        44%
        0.3
        402082_ITS_KP8LM_ACCACATA-ACGACGTG_S36_L001_R2
        89.0%
        49%
        0.3
        402083_ITS_KP8LM_ACCACATA-ATATACAC_S37_L001_R2
        23.0%
        48%
        0.0
        402084_ITS_KP8LM_ACCACATA-CGTCGCTA_S38_L001_R2
        84.0%
        50%
        0.1
        402085_ITS_KP8LM_ACCACATA-CTAGAGCT_S39_L001_R2
        86.9%
        45%
        0.3
        402086_ITS_KP8LM_ACCACATA-GCTCTAGT_S40_L001_R2
        87.3%
        48%
        0.3
        402087_ITS_KP8LM_GCAGGATA-CGTCGCTA_S41_L001_R2
        88.4%
        49%
        0.3
        402088_ITS_KP8LM_ACCACATA-TGCGTACG_S42_L001_R2
        88.1%
        48%
        0.2
        402089_ITS_KP8LM_ACCACATA-TAGTGTAG_S43_L001_R2
        69.2%
        46%
        0.0
        402090_ITS_KP8LM_GCAGGATA-GCTCTAGT_S44_L001_R2
        90.3%
        45%
        0.4
        402091_ITS_KP8LM_GCAGGATA-GACACTGA_S45_L001_R2
        86.8%
        44%
        0.1
        402092_ITS_KP8LM_AACACAAG-CGTCGCTA_S46_L001_R2
        89.7%
        46%
        0.2
        402093_ITS_KP8LM_GCAGGATA-TGCGTACG_S47_L001_R2
        89.9%
        50%
        0.3
        402094_ITS_KP8LM_AACACAAG-GCTCTAGT_S48_L001_R2
        87.3%
        47%
        0.3
        402095_ITS_KP8LM_GCAGGATA-TAGTGTAG_S49_L001_R2
        84.3%
        43%
        0.0
        402096_ITS_KP8LM_AACACAAG-TGCGTACG_S50_L001_R2
        83.9%
        49%
        0.1
        402097_ITS_KP8LM_AACACAAG-TAGTGTAG_S51_L001_R2
        84.0%
        48%
        0.1
        402098_ITS_KP8LM_AATGTCCG-ACGACGTG_S52_L001_R2
        81.6%
        50%
        0.0
        402099_ITS_KP8LM_AATGTCCG-ATATACAC_S53_L001_R2
        88.8%
        48%
        0.2
        402100_ITS_KP8LM_AATGTCCG-CGTCGCTA_S54_L001_R2
        85.8%
        50%
        0.1
        402101_ITS_KP8LM_CGTCCGAA-ACGACGTG_S55_L001_R2
        88.2%
        46%
        0.2
        402102_ITS_KP8LM_AATGTCCG-GCTCTAGT_S56_L001_R2
        83.7%
        51%
        0.1
        402103_ITS_KP8LM_AATGTCCG-GACACTGA_S57_L001_R2
        60.9%
        50%
        0.0
        402104_ITS_KP8LM_AATGTCCG-TGCGTACG_S58_L001_R2
        85.1%
        49%
        0.1
        402105_ITS_KP8LM_AATGTCCG-TAGTGTAG_S59_L001_R2
        87.4%
        48%
        0.2
        402106_ITS_KP8LM_TACTTCGC-ACGACGTG_S60_L001_R2
        75.1%
        50%
        0.0
        402107_ITS_KP8LM_TACTTCGC-ATATACAC_S61_L001_R2
        88.4%
        50%
        0.4
        402108_ITS_KP8LM_TACTTCGC-CGTCGCTA_S62_L001_R2
        80.5%
        47%
        0.1
        402109_ITS_KP8LM_TACTTCGC-CTAGAGCT_S63_L001_R2
        83.5%
        50%
        0.1
        402110_ITS_KP8LM_TACTTCGC-GCTCTAGT_S64_L001_R2
        82.5%
        47%
        0.1
        402111_ITS_KP8LM_CGTCCGAA-CGTCGCTA_S65_L001_R2
        87.1%
        50%
        0.1
        402112_ITS_KP8LM_TACTTCGC-TGCGTACG_S66_L001_R2
        81.0%
        46%
        0.1
        402113_ITS_KP8LM_TACTTCGC-TAGTGTAG_S67_L001_R2
        88.5%
        49%
        0.2
        402114_ITS_KP8LM_GCTTCGGT-ACGACGTG_S68_L001_R2
        80.5%
        46%
        0.0
        402115_ITS_KP8LM_GCTTCGGT-ATATACAC_S69_L001_R2
        83.0%
        49%
        0.0
        402116_ITS_KP8LM_GCTTCGGT-CGTCGCTA_S70_L001_R2
        81.6%
        48%
        0.0
        402117_ITS_KP8LM_GCTTCGGT-CTAGAGCT_S71_L001_R2
        83.7%
        48%
        0.0
        402118_ITS_KP8LM_GCTTCGGT-GCTCTAGT_S72_L001_R2
        79.3%
        48%
        0.0
        402119_ITS_KP8LM_GCTTCGGT-GACACTGA_S73_L001_R2
        78.9%
        47%
        0.0
        402120_ITS_KP8LM_GCTTCGGT-TGCGTACG_S74_L001_R2
        81.0%
        44%
        0.0
        402121_ITS_KP8LM_GCTTCGGT-TAGTGTAG_S75_L001_R2
        82.7%
        47%
        0.0
        402122_ITS_KP8LM_CTTACACC-ACGACGTG_S76_L001_R2
        77.4%
        47%
        0.0
        402123_ITS_KP8LM_CTTACACC-ATATACAC_S77_L001_R2
        79.4%
        48%
        0.1
        402124_ITS_KP8LM_CTTACACC-CGTCGCTA_S78_L001_R2
        77.6%
        48%
        0.0
        402125_ITS_KP8LM_CTTACACC-CTAGAGCT_S79_L001_R2
        80.6%
        47%
        0.0
        402126_ITS_KP8LM_CTTACACC-GCTCTAGT_S80_L001_R2
        80.2%
        48%
        0.0
        402127_ITS_KP8LM_CTTACACC-GACACTGA_S81_L001_R2
        80.6%
        46%
        0.1
        402128_ITS_KP8LM_CTTACACC-TGCGTACG_S82_L001_R2
        54.3%
        48%
        0.0
        402129_ITS_KP8LM_CTTACACC-TAGTGTAG_S83_L001_R2
        84.9%
        49%
        0.1
        402130_ITS_KP8LM_TGACCTCC-ACGACGTG_S84_L001_R2
        80.9%
        47%
        0.1
        402131_ITS_KP8LM_TGACCTCC-ATATACAC_S85_L001_R2
        79.2%
        48%
        0.1
        402132_ITS_KP8LM_TGACCTCC-CGTCGCTA_S86_L001_R2
        80.1%
        48%
        0.1
        402133_ITS_KP8LM_TGACCTCC-CTAGAGCT_S87_L001_R2
        80.0%
        49%
        0.1
        402134_ITS_KP8LM_TGACCTCC-GCTCTAGT_S88_L001_R2
        78.7%
        48%
        0.1
        402135_ITS_KP8LM_TGACCTCC-GACACTGA_S89_L001_R2
        79.8%
        49%
        0.1
        402136_ITS_KP8LM_TGACCTCC-TGCGTACG_S90_L001_R2
        76.3%
        48%
        0.1
        402137_ITS_KP8LM_TGACCTCC-TAGTGTAG_S91_L001_R2
        78.0%
        48%
        0.1
        402138_ITS_KP8LM_ACAAGGAG-ACGACGTG_S92_L001_R2
        79.2%
        48%
        0.0
        402139_ITS_KP8LM_ACAAGGAG-ATATACAC_S93_L001_R2
        83.3%
        49%
        0.1
        402140_ITS_KP8LM_ACAAGGAG-CGTCGCTA_S94_L001_R2
        79.8%
        49%
        0.0
        402141_ITS_KP8LM_ACAAGGAG-CTAGAGCT_S95_L001_R2
        80.6%
        49%
        0.0
        402142_ITS_KP8LM_ACAAGGAG-GCTCTAGT_S96_L001_R2
        82.3%
        48%
        0.1
        402143_ITS_KP8LM_TCTGTTGC-CTAGAGCT_S97_L001_R2
        88.3%
        45%
        0.4
        402144_ITS_KP8LM_TCTGTTGC-GCTCTAGT_S98_L001_R2
        87.4%
        50%
        0.1
        402145_ITS_KP8LM_TCTGTTGC-GACACTGA_S99_L001_R2
        85.1%
        49%
        0.1
        402146_ITS_KP69D_TATCAGGT-ACGACGTG_S94_L001_R2
        84.3%
        48%
        0.1
        402147_ITS_KP69D_TATCAGGT-ATATACAC_S95_L001_R2
        83.3%
        49%
        0.2
        402148_ITS_KP69D_TATCAGGT-CGTCGCTA_S96_L001_R2
        87.9%
        50%
        0.2
        402149_ITS_KP69D_TATCAGGT-CTAGAGCT_S97_L001_R2
        88.0%
        49%
        0.2
        402150_ITS_KP69D_TATCAGGT-GCTCTAGT_S98_L001_R2
        82.5%
        48%
        0.1
        402151_ITS_KP69D_TATCAGGT-GACACTGA_S99_L001_R2
        80.5%
        48%
        0.1
        402152_ITS_KP69D_TATCAGGT-TGCGTACG_S100_L001_R2
        83.8%
        48%
        0.1
        402153_ITS_KP69D_TATCAGGT-TAGTGTAG_S101_L001_R2
        82.5%
        48%
        0.1
        402154_ITS_KP69D_TGTAATTG-ACGACGTG_S102_L001_R2
        82.2%
        47%
        0.1
        402155_ITS_KP69D_TGTAATTG-ATATACAC_S103_L001_R2
        82.1%
        47%
        0.1
        402156_ITS_KP69D_TGTAATTG-CGTCGCTA_S104_L001_R2
        83.7%
        48%
        0.1
        402157_ITS_KP69D_TGTAATTG-CTAGAGCT_S105_L001_R2
        84.8%
        49%
        0.1
        402158_ITS_KP69D_TGTAATTG-GCTCTAGT_S106_L001_R2
        84.3%
        49%
        0.1
        402159_ITS_KP69D_TGTAATTG-GACACTGA_S107_L001_R2
        82.7%
        48%
        0.1
        402160_ITS_KP69D_TGTAATTG-TGCGTACG_S108_L001_R2
        83.0%
        48%
        0.1
        402161_ITS_KP69D_TGTAATTG-TAGTGTAG_S109_L001_R2
        82.0%
        48%
        0.1
        402162_ITS_KP69D_AATGGAGC-ACGACGTG_S110_L001_R2
        83.0%
        49%
        0.1
        402163_ITS_KP8LM_AATGGAGC-ATATACAC_S100_L001_R2
        84.2%
        47%
        0.1
        402164_ITS_KP8LM_AATGGAGC-CGTCGCTA_S101_L001_R2
        84.7%
        51%
        0.1
        402165_ITS_KP8LM_AATGGAGC-CTAGAGCT_S102_L001_R2
        83.6%
        50%
        0.1
        59000_ITS_KP8LM_AGCTTGAC-ACGACGTG_S103_L001_R2
        83.0%
        48%
        0.1
        59001_ITS_KP8LM_AGCTTGAC-ATATACAC_S104_L001_R2
        83.8%
        50%
        0.1
        59002_ITS_KP8LM_AGCTTGAC-CGTCGCTA_S105_L001_R2
        84.7%
        51%
        0.1
        59003_ITS_KP8LM_AGCTTGAC-CTAGAGCT_S106_L001_R2
        88.5%
        47%
        0.1
        59004_ITS_KP8LM_AGCTTGAC-GCTCTAGT_S107_L001_R2
        82.5%
        48%
        0.1
        59005_ITS_KP8LM_AGCTTGAC-GACACTGA_S108_L001_R2
        84.3%
        49%
        0.1
        59006_ITS_KP8LM_AGCTTGAC-TGCGTACG_S109_L001_R2
        88.3%
        50%
        0.1
        59007_ITS_KP8LM_AGCTTGAC-TAGTGTAG_S110_L001_R2
        79.3%
        51%
        0.0
        ATCC1010MOCK_ITS_KP69D_AATGGAGC-GCTCTAGT_S111_L001_R2
        91.2%
        49%
        0.2
        ATCC1010MOCK_ITS_KP69D_ACAAGGAG-GACACTGA_S112_L001_R2
        90.6%
        48%
        0.2
        ATCC1010MOCK_ITS_KP8LM_ACAAGGAG-GACACTGA_S111_L001_R2
        89.9%
        48%
        0.1
        ATCC1010MOCK_ITS_KP8LM_CGTCCGAA-CTAGAGCT_S112_L001_R2
        91.2%
        49%
        0.1
        No_Template_Control_ITS_KP69D_AATGGAGC-TGCGTACG_S113_L001_R2
        57.8%
        47%
        0.0
        No_Template_Control_ITS_KP69D_ACAAGGAG-TAGTGTAG_S114_L001_R2
        53.8%
        44%
        0.0
        No_Template_Control_ITS_KP8LM_ACAAGGAG-TAGTGTAG_S113_L001_R2
        71.9%
        45%
        0.0
        No_Template_Control_ITS_KP8LM_CGTCCGAA-GACACTGA_S114_L001_R2
        58.6%
        46%
        0.0
        Soil_DNA_ITS_KP69D_AATGGAGC-GACACTGA_S115_L001_R2
        82.8%
        46%
        0.4
        Soil_DNA_ITS_KP69D_ACAAGGAG-TGCGTACG_S116_L001_R2
        83.2%
        45%
        0.3
        Soil_DNA_ITS_KP8LM_ACAAGGAG-TGCGTACG_S115_L001_R2
        84.3%
        46%
        0.2
        Soil_DNA_ITS_KP8LM_CGTCCGAA-GCTCTAGT_S116_L001_R2
        84.1%
        47%
        0.2

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        Sequence Length Distribution

        The distribution of fragment sizes (read lengths) found. See the FastQC help

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        No samples found with any adapter contamination > 0.1%

        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

        loading..

        MultiQC v1.13 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.